Starting phenix.real_space_refine on Sat Apr 26 22:35:32 2025 by dcliebschner =============================================================================== Processing files: ------------------------------------------------------------------------------- Found model, /net/cci-nas-00/data/ceres_data/9mek_48216/04_2025/9mek_48216.cif Found real_map, /net/cci-nas-00/data/ceres_data/9mek_48216/04_2025/9mek_48216.map Processing PHIL parameters: ------------------------------------------------------------------------------- Adding command-line PHIL: ------------------------- refinement.macro_cycles=10 scattering_table=electron resolution=2.85 write_initial_geo_file=False Final processed PHIL parameters: ------------------------------------------------------------------------------- data_manager { real_map_files = "/net/cci-nas-00/data/ceres_data/9mek_48216/04_2025/9mek_48216.map" default_real_map = "/net/cci-nas-00/data/ceres_data/9mek_48216/04_2025/9mek_48216.map" model { file = "/net/cci-nas-00/data/ceres_data/9mek_48216/04_2025/9mek_48216.cif" } default_model = "/net/cci-nas-00/data/ceres_data/9mek_48216/04_2025/9mek_48216.cif" } resolution = 2.85 write_initial_geo_file = False refinement { macro_cycles = 10 } qi { qm_restraints { package { program = *test } } } Starting job =============================================================================== ------------------------------------------------------------------------------- Citation: ********* Afonine PV, Poon BK, Read RJ, Sobolev OV, Terwilliger TC, Urzhumtsev A, Adams PD. (2018) Real-space refinement in PHENIX for cryo-EM and crystallography. Acta Cryst. D74:531-544. Validating inputs Origin is already at (0, 0, 0), no shifts will be applied ------------------------------------------------------------------------------- Processing inputs ***************** Set random seed Set to: 0 Set model cs if undefined Decide on map wrapping Map wrapping is set to: False Normalize map: mean=0, sd=1 Input map: mean= -0.001 sd= 0.062 Set stop_for_unknowns flag Set to: True Assert model is a single copy model Assert all atoms have isotropic ADPs Construct map_model_manager Extract box with map and model Check model and map are aligned Set scattering table Set to: electron Number of scattering types: 5 Type Number sf(0) Gaussians K 4 8.98 5 S 12 5.16 5 C 2396 2.51 5 N 576 2.21 5 O 606 1.98 5 sf(0) = scattering factor at diffraction angle 0. Process input model Monomer Library directory: "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/chem_data/mon_lib" Total number of atoms: 3594 Number of models: 1 Model: "" Number of chains: 3 Chain: "A" Number of atoms: 1788 Number of conformers: 1 Conformer: "" Number of residues, atoms: 239, 1788 Classifications: {'peptide': 239} Incomplete info: {'truncation_to_alanine': 7} Link IDs: {'PTRANS': 9, 'TRANS': 229} Chain breaks: 2 Unresolved non-hydrogen bonds: 35 Unresolved non-hydrogen angles: 42 Unresolved non-hydrogen dihedrals: 28 Planarities with less than four sites: {'GLU:plan': 2, 'ARG:plan': 4, 'ASP:plan': 1} Unresolved non-hydrogen planarities: 31 Chain: "A" Number of atoms: 11 Number of conformers: 1 Conformer: "" Number of residues, atoms: 5, 11 Unusual residues: {' K': 4, 'HP6': 1} Classifications: {'undetermined': 5} Link IDs: {None: 4} Chain: "B" Number of atoms: 7 Number of conformers: 1 Conformer: "" Number of residues, atoms: 1, 7 Unusual residues: {'HP6': 1} Classifications: {'undetermined': 1} Restraints were copied for chains: B Time building chain proxies: 4.09, per 1000 atoms: 1.14 Number of scatterers: 3594 At special positions: 0 Unit cell: (71.775, 61.625, 89.9, 90, 90, 90) Space group: P 1 (No. 1) Number of sites at special positions: 0 Number of scattering types: 5 Type Number sf(0) K 4 19.00 S 12 16.00 O 606 8.00 N 576 7.00 C 2396 6.00 sf(0) = scattering factor at diffraction angle 0. Number of disulfides: simple=1, symmetry=0 Simple disulfide: pdb=" SG CYS A 53 " - pdb=" SG CYS B 53 " distance=2.03 Automatic linking Parameters for automatic linking Linking & cutoffs Metal : Auto - 3.00 Amino acid : True - 1.90 Carbohydrate : True - 1.99 Ligands : True - 1.99 Small molecules : False - 1.98 Amino acid - RNA/DNA : False Number of custom bonds: simple=0, symmetry=0 Time building additional restraints: 0.97 Conformation dependent library (CDL) restraints added in 431.5 milliseconds 932 Ramachandran restraints generated. 466 Oldfield, 0 Emsley, 466 emsley8k and 0 Phi/Psi/2. Adding C-beta torsion restraints... Number of C-beta restraints generated: 892 Finding SS restraints... Secondary structure from input PDB file: 17 helices and 0 sheets defined 77.4% alpha, 0.0% beta 0 base pairs and 0 stacking pairs defined. Time for finding SS restraints: 0.29 Creating SS restraints... Processing helix chain 'A' and resid 7 through 51 Proline residue: A 31 - end of helix Processing helix chain 'A' and resid 55 through 69 Processing helix chain 'A' and resid 72 through 78 removed outlier: 3.796A pdb=" N VAL A 76 " --> pdb=" O LEU A 72 " (cutoff:3.500A) Processing helix chain 'A' and resid 92 through 105 Processing helix chain 'A' and resid 116 through 151 Proline residue: A 132 - end of helix removed outlier: 3.592A pdb=" N HIS A 151 " --> pdb=" O LEU A 147 " (cutoff:3.500A) Processing helix chain 'A' and resid 167 through 195 removed outlier: 3.608A pdb=" N TRP A 171 " --> pdb=" O ARG A 167 " (cutoff:3.500A) removed outlier: 3.752A pdb=" N LEU A 186 " --> pdb=" O THR A 182 " (cutoff:3.500A) removed outlier: 4.656A pdb=" N VAL A 187 " --> pdb=" O VAL A 183 " (cutoff:3.500A) Proline residue: A 188 - end of helix Processing helix chain 'A' and resid 200 through 213 Processing helix chain 'A' and resid 230 through 264 Processing helix chain 'A' and resid 267 through 271 Processing helix chain 'B' and resid 8 through 51 Proline residue: B 31 - end of helix Processing helix chain 'B' and resid 55 through 69 Processing helix chain 'B' and resid 72 through 78 removed outlier: 3.796A pdb=" N VAL B 76 " --> pdb=" O LEU B 72 " (cutoff:3.500A) Processing helix chain 'B' and resid 92 through 105 Processing helix chain 'B' and resid 116 through 151 Proline residue: B 132 - end of helix removed outlier: 3.592A pdb=" N HIS B 151 " --> pdb=" O LEU B 147 " (cutoff:3.500A) Processing helix chain 'B' and resid 200 through 213 Processing helix chain 'B' and resid 230 through 264 Processing helix chain 'B' and resid 267 through 271 280 hydrogen bonds defined for protein. 840 hydrogen bond angles defined for protein. Restraints generated for nucleic acids: 0 hydrogen bonds 0 hydrogen bond angles 0 basepair planarities 0 basepair parallelities 0 stacking parallelities Total time for adding SS restraints: 1.06 Time building geometry restraints manager: 1.11 seconds NOTE: a complete listing of the restraints can be obtained by requesting output of .geo file. Histogram of bond lengths: 1.23 - 1.34: 1096 1.34 - 1.46: 832 1.46 - 1.58: 1726 1.58 - 1.69: 0 1.69 - 1.81: 16 Bond restraints: 3670 Sorted by residual: bond pdb=" CA PRO A 226 " pdb=" C PRO A 226 " ideal model delta sigma weight residual 1.527 1.520 0.007 1.07e-02 8.73e+03 4.60e-01 bond pdb=" CA PRO B 226 " pdb=" C PRO B 226 " ideal model delta sigma weight residual 1.527 1.520 0.007 1.07e-02 8.73e+03 4.60e-01 bond pdb=" CA PRO B 226 " pdb=" CB PRO B 226 " ideal model delta sigma weight residual 1.530 1.537 -0.006 1.03e-02 9.43e+03 3.64e-01 bond pdb=" CA PRO A 226 " pdb=" CB PRO A 226 " ideal model delta sigma weight residual 1.530 1.537 -0.006 1.03e-02 9.43e+03 3.64e-01 bond pdb=" N PRO A 226 " pdb=" CA PRO A 226 " ideal model delta sigma weight residual 1.479 1.470 0.010 1.71e-02 3.42e+03 3.24e-01 ... (remaining 3665 not shown) Histogram of bond angle deviations from ideal: 0.00 - 1.01: 4780 1.01 - 2.02: 176 2.02 - 3.03: 44 3.03 - 4.05: 6 4.05 - 5.06: 14 Bond angle restraints: 5020 Sorted by residual: angle pdb=" N PHE A 185 " pdb=" CA PHE A 185 " pdb=" C PHE A 185 " ideal model delta sigma weight residual 114.75 110.69 4.06 1.26e+00 6.30e-01 1.04e+01 angle pdb=" N PHE B 185 " pdb=" CA PHE B 185 " pdb=" C PHE B 185 " ideal model delta sigma weight residual 114.75 110.69 4.06 1.26e+00 6.30e-01 1.04e+01 angle pdb=" CA PHE B 185 " pdb=" C PHE B 185 " pdb=" N LEU B 186 " ideal model delta sigma weight residual 119.80 117.04 2.76 1.34e+00 5.57e-01 4.25e+00 angle pdb=" CA PHE A 185 " pdb=" C PHE A 185 " pdb=" N LEU A 186 " ideal model delta sigma weight residual 119.80 117.04 2.76 1.34e+00 5.57e-01 4.25e+00 angle pdb=" N GLY B 130 " pdb=" CA GLY B 130 " pdb=" C GLY B 130 " ideal model delta sigma weight residual 113.18 117.61 -4.43 2.37e+00 1.78e-01 3.49e+00 ... (remaining 5015 not shown) Histogram of dihedral angle deviations from ideal: 0.00 - 12.48: 1758 12.48 - 24.97: 221 24.97 - 37.45: 98 37.45 - 49.93: 26 49.93 - 62.41: 6 Dihedral angle restraints: 2109 sinusoidal: 727 harmonic: 1382 Sorted by residual: dihedral pdb=" CB ARG A 27 " pdb=" CG ARG A 27 " pdb=" CD ARG A 27 " pdb=" NE ARG A 27 " ideal model delta sinusoidal sigma weight residual -180.00 -127.27 -52.73 3 1.50e+01 4.44e-03 9.14e+00 dihedral pdb=" CB ARG B 27 " pdb=" CG ARG B 27 " pdb=" CD ARG B 27 " pdb=" NE ARG B 27 " ideal model delta sinusoidal sigma weight residual -180.00 -127.27 -52.73 3 1.50e+01 4.44e-03 9.14e+00 dihedral pdb=" N LEU A 43 " pdb=" CA LEU A 43 " pdb=" CB LEU A 43 " pdb=" CG LEU A 43 " ideal model delta sinusoidal sigma weight residual -180.00 -129.61 -50.39 3 1.50e+01 4.44e-03 8.89e+00 ... (remaining 2106 not shown) Histogram of chiral volume deviations from ideal: 0.000 - 0.025: 398 0.025 - 0.049: 146 0.049 - 0.073: 62 0.073 - 0.098: 14 0.098 - 0.122: 14 Chirality restraints: 634 Sorted by residual: chirality pdb=" CB THR A 42 " pdb=" CA THR A 42 " pdb=" OG1 THR A 42 " pdb=" CG2 THR A 42 " both_signs ideal model delta sigma weight residual False 2.55 2.43 0.12 2.00e-01 2.50e+01 3.72e-01 chirality pdb=" CB THR B 42 " pdb=" CA THR B 42 " pdb=" OG1 THR B 42 " pdb=" CG2 THR B 42 " both_signs ideal model delta sigma weight residual False 2.55 2.43 0.12 2.00e-01 2.50e+01 3.72e-01 chirality pdb=" CA THR B 106 " pdb=" N THR B 106 " pdb=" C THR B 106 " pdb=" CB THR B 106 " both_signs ideal model delta sigma weight residual False 2.53 2.41 0.12 2.00e-01 2.50e+01 3.48e-01 ... (remaining 631 not shown) Planarity restraints: 606 Sorted by residual: delta sigma weight rms_deltas residual plane pdb=" C ALA A 225 " -0.018 5.00e-02 4.00e+02 2.71e-02 1.17e+00 pdb=" N PRO A 226 " 0.047 5.00e-02 4.00e+02 pdb=" CA PRO A 226 " -0.014 5.00e-02 4.00e+02 pdb=" CD PRO A 226 " -0.015 5.00e-02 4.00e+02 delta sigma weight rms_deltas residual plane pdb=" C ALA B 225 " 0.018 5.00e-02 4.00e+02 2.71e-02 1.17e+00 pdb=" N PRO B 226 " -0.047 5.00e-02 4.00e+02 pdb=" CA PRO B 226 " 0.014 5.00e-02 4.00e+02 pdb=" CD PRO B 226 " 0.015 5.00e-02 4.00e+02 delta sigma weight rms_deltas residual plane pdb=" CA VAL B 181 " -0.005 2.00e-02 2.50e+03 1.06e-02 1.12e+00 pdb=" C VAL B 181 " 0.018 2.00e-02 2.50e+03 pdb=" O VAL B 181 " -0.007 2.00e-02 2.50e+03 pdb=" N THR B 182 " -0.006 2.00e-02 2.50e+03 ... (remaining 603 not shown) Histogram of nonbonded interaction distances: 2.31 - 2.87: 1335 2.87 - 3.44: 4354 3.44 - 4.01: 6694 4.01 - 4.57: 9960 4.57 - 5.14: 13224 Nonbonded interactions: 35567 Sorted by model distance: nonbonded pdb=" O PHE A 209 " pdb=" OG SER A 213 " model vdw 2.306 3.040 nonbonded pdb=" O PHE B 209 " pdb=" OG SER B 213 " model vdw 2.306 3.040 nonbonded pdb=" OE2 GLU A 29 " pdb=" N LEU B 115 " model vdw 2.311 3.120 nonbonded pdb=" N LEU A 115 " pdb=" OE2 GLU B 29 " model vdw 2.311 3.120 nonbonded pdb=" O SER B 101 " pdb=" OG1 THR B 105 " model vdw 2.318 3.040 ... (remaining 35562 not shown) NOTE: a complete listing of the restraints can be obtained by requesting output of .geo file. Find NCS groups from input model Time spend for trying shortcut: 0.00 Found NCS groups: ncs_group { reference = (chain 'A' and resid 7 through 271) selection = (chain 'B' and resid 7 through 271) } Set up NCS constraints No NCS constraints will be used in refinement. Set refine NCS operators Adjust number of macro_cycles Number of macro_cycles: 10 Reset NCS operators Extract rigid body selections Check and reset occupancies Occupancies: min=0.28 max=1.00 mean=1.00 Load rotamer database and sin/cos tables Set ADP refinement strategy ADPs will be refined as individual isotropic Make a string to write initial .geo file Internal consistency checks Time: Set random seed: 0.000 Set model cs if undefined: 0.000 Decide on map wrapping: 0.000 Normalize map: mean=0, sd=1: 2.760 Set stop_for_unknowns flag: 0.000 Assert model is a single copy model: 0.000 Assert all atoms have isotropic ADPs: 0.000 Construct map_model_manager: 0.010 Extract box with map and model: 0.160 Check model and map are aligned: 0.030 Set scattering table: 0.030 Process input model: 14.860 Find NCS groups from input model: 0.150 Set up NCS constraints: 0.020 Set refine NCS operators: 0.000 Adjust number of macro_cycles: 0.000 Reset NCS operators: 0.000 Extract rigid body selections: 0.000 Check and reset occupancies: 0.000 Load rotamer database and sin/cos tables:15.300 Set ADP refinement strategy: 0.000 Make a string to write initial .geo file:0.000 Internal consistency checks: 0.000 Total: 33.320 ------------------------------------------------------------------------------- Set refinement monitor ********************** ------------------------------------------------------------------------------- Setup refinement engine *********************** ------------------------------------------------------------------------------- Overall statistics ****************** model-to-map fit, CC_mask: 0.8039 moved from start: 0.0000 Geometry Restraints Library: GeoStd + Monomer Library + CDL v1.2 Deviations from Ideal Values - rmsd, rmsZ for bonds and angles. Bond : 0.003 0.024 3671 Z= 0.126 Angle : 0.526 5.058 5022 Z= 0.280 Chirality : 0.034 0.122 634 Planarity : 0.003 0.027 606 Dihedral : 14.272 62.411 1214 Min Nonbonded Distance : 2.306 Molprobity Statistics. All-atom Clashscore : 6.84 Ramachandran Plot: Outliers : 0.00 % Allowed : 2.15 % Favored : 97.85 % Rotamer: Outliers : 1.68 % Allowed : 20.11 % Favored : 78.21 % Cbeta Deviations : 0.00 % Peptide Plane: Cis-proline : 0.00 % Cis-general : 0.00 % Twisted Proline : 0.00 % Twisted General : 0.00 % Rama-Z (Ramachandran plot Z-score): Interpretation: bad |Rama-Z| > 3; suspicious 2 < |Rama-Z| < 3; good |Rama-Z| < 2. Scores for whole/helix/sheet/loop are scaled independently; therefore, the values are not related in a simple manner. whole: 3.48 (0.40), residues: 466 helix: 2.94 (0.25), residues: 382 sheet: None (None), residues: 0 loop : -1.00 (0.81), residues: 84 Max deviation from planes: Type MaxDev MeanDev LineInFile TRP 0.007 0.001 TRP A 199 HIS 0.006 0.001 HIS B 258 PHE 0.009 0.002 PHE A 93 TYR 0.008 0.001 TYR B 206 ARG 0.001 0.000 ARG B 44 Details of bonding type rmsd hydrogen bonds : bond 0.09847 ( 280) hydrogen bonds : angle 4.68874 ( 840) SS BOND : bond 0.00131 ( 1) SS BOND : angle 0.07131 ( 2) covalent geometry : bond 0.00275 ( 3670) covalent geometry : angle 0.52631 ( 5020) *********************** REFINEMENT MACRO_CYCLE 1 OF 10 ************************ ------------------------------------------------------------------------------- Update Rama plot phi/psi targets (oldfield only) ************************************************ 932 Ramachandran restraints generated. 466 Oldfield, 0 Emsley, 466 emsley8k and 0 Phi/Psi/2. ------------------------------------------------------------------------------- Re-set Ramachandran plot restraints *********************************** favored: oldfield allowed: oldfield outlier: oldfield 932 Ramachandran restraints generated. 466 Oldfield, 0 Emsley, 466 emsley8k and 0 Phi/Psi/2. ------------------------------------------------------------------------------- Optimize residue side-chains **************************** Evaluate side-chains 36 residues out of total 372 (non-[ALA,GLY,PRO]) need to be fit. rotamer outliers: 6 poor density : 30 time to evaluate : 0.410 Fit side-chains REVERT: A 11 LEU cc_start: 0.8155 (OUTLIER) cc_final: 0.7605 (mm) REVERT: B 11 LEU cc_start: 0.8157 (OUTLIER) cc_final: 0.7608 (mm) outliers start: 6 outliers final: 2 residues processed: 36 average time/residue: 0.5454 time to fit residues: 21.2976 Evaluate side-chains 32 residues out of total 372 (non-[ALA,GLY,PRO]) need to be fit. rotamer outliers: 4 poor density : 28 time to evaluate : 0.346 ------------------------------------------------------------------------------- Set rotamer restraints ********************** Chi-restraints excluded: chain A residue 11 LEU Chi-restraints excluded: chain A residue 269 ILE Chi-restraints excluded: chain B residue 11 LEU Chi-restraints excluded: chain B residue 269 ILE Rotamers are restrained with sigma=5.00 ------------------------------------------------------------------------------- XYZ refinement ************** Traceback (most recent call last): File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/build/../modules/phenix/phenix/command_line/real_space_refine.py", line 8, in run_program(real_space_refine.Program) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/iotbx/cli_parser.py", line 994, in run_program task.run() File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/phenix/phenix/programs/real_space_refine.py", line 210, in run log = self.logger) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/phenix/phenix/refinement/rsr/wrappers.py", line 58, in __init__ log = log) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/phenix/phenix/refinement/macro_cycle_real_space.py", line 306, in __init__ self.caller(self.refine_xyz) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/phenix/phenix/refinement/macro_cycle_real_space.py", line 321, in caller func() File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/phenix/phenix/refinement/macro_cycle_real_space.py", line 702, in refine_xyz self.minimization_no_ncs() File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/phenix/phenix/refinement/macro_cycle_real_space.py", line 767, in minimization_no_ncs log = self.log) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/mmtbx/refinement/real_space/individual_sites.py", line 51, in __init__ gradients_method = gradients_method) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/mmtbx/refinement/real_space/weight.py", line 87, in __init__ rms_angles_limit = rms_angles_limit) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/mmtbx/refinement/real_space/individual_sites.py", line 368, in refine rms_angles_limit = rms_angles_limit) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/mmtbx/refinement/real_space/individual_sites.py", line 235, in __init__ weight = weight) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/mmtbx/refinement/real_space/individual_sites.py", line 112, in refine states_collector = self.states_accumulator) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/cctbx/maptbx/real_space_refinement_simple.py", line 174, in __init__ exception_handling_params=lbfgs_exception_handling_params) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/scitbx/lbfgs/__init__.py", line 278, in run line_search) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/scitbx/lbfgs/__init__.py", line 131, in run_c_plus_plus f, g = target_evaluator.compute_functional_and_gradients() File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/cctbx/maptbx/real_space_refinement_simple.py", line 245, in compute_functional_and_gradients compute_gradients=True) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/cctbx/geometry_restraints/manager.py", line 1525, in energies_sites flags=flags, sites_cart=sites_cart, site_labels=site_labels) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/cctbx/geometry_restraints/manager.py", line 1427, in pair_proxies check_bonded_distance_cutoff(sites_frac=sites_frac) File "/net/cci-filer2/raid1/xp/phenix/phenix-dev-5669/modules/cctbx_project/cctbx/geometry_restraints/manager.py", line 1308, in check_bonded_distance_cutoff raise RuntimeError(msg) RuntimeError: Bond distance > max_reasonable_bond_distance: 55.0899 > 50: distance: 78 - 107: 6.186 distance: 99 - 100: 3.899 distance: 100 - 101: 7.755 distance: 100 - 103: 4.495 distance: 101 - 102: 17.714 distance: 101 - 107: 13.131 distance: 103 - 104: 13.596 distance: 104 - 105: 12.820 distance: 107 - 108: 4.221 distance: 108 - 109: 6.536 distance: 108 - 111: 6.686 distance: 109 - 110: 7.496 distance: 109 - 113: 5.651 distance: 111 - 112: 8.879 distance: 113 - 114: 6.803 distance: 114 - 115: 3.258 distance: 114 - 117: 6.474 distance: 115 - 116: 5.497 distance: 115 - 120: 6.717 distance: 117 - 118: 6.571 distance: 117 - 119: 6.484 distance: 120 - 121: 10.721 distance: 121 - 122: 3.342 distance: 121 - 124: 6.166 distance: 122 - 123: 8.151 distance: 122 - 128: 11.168 distance: 124 - 125: 7.287 distance: 124 - 126: 8.035 distance: 125 - 127: 9.335 distance: 128 - 129: 8.875 distance: 129 - 130: 5.149 distance: 130 - 131: 34.990 distance: 130 - 132: 18.666 distance: 132 - 133: 31.192 distance: 133 - 134: 16.830 distance: 133 - 136: 17.125 distance: 134 - 135: 20.563 distance: 134 - 140: 32.383 distance: 136 - 137: 25.524 distance: 137 - 138: 10.762 distance: 137 - 139: 12.032 distance: 140 - 141: 9.382 distance: 141 - 142: 13.008 distance: 142 - 143: 19.931 distance: 142 - 144: 35.647 distance: 144 - 145: 13.428 distance: 145 - 146: 8.904 distance: 145 - 148: 10.582 distance: 146 - 147: 17.103 distance: 146 - 152: 5.911 distance: 148 - 149: 7.467 distance: 149 - 150: 9.341 distance: 149 - 151: 7.913 distance: 152 - 153: 3.245 distance: 153 - 154: 4.076 distance: 153 - 156: 4.391 distance: 154 - 155: 4.321 distance: 157 - 158: 3.630 distance: 157 - 159: 4.511 distance: 160 - 162: 4.207 distance: 161 - 162: 3.786 distance: 164 - 165: 4.839 distance: 165 - 168: 3.727 distance: 168 - 169: 3.736 distance: 168 - 170: 5.073